版权说明 帮助中心 进入管理中心
首页 > 成果 > 详情

Poly(ethylene glycol)-block-polyethylenimine copolymers as carriers for gene delivery: Effects of PEG molecular weight and PEGylation degree

SCI-EEIMedline
WOS被引频次:81
认领
导出
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Zhang, Xuan;Pan, Shi-Rong;Hu, Hai-Mei;Wu, Gui-Fu;Feng, Min;Zhang, Wei;Luo, Xin
通讯作者:
Pan, S.-R.(gzpshr@163.com)
作者机构:
[Feng, Min; Luo, Xin] School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510080, China
[Pan, Shi-Rong; Zhang, Xuan; Zhang, Wei; Wu, Gui-Fu] First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China
[Hu, Hai-Mei] School of Life Science and Biopharmacology, Guang Dong Pharmaceutical University, Guangzhou 510006, China
通讯机构:
[Pan, SR] Sun Yat Sen Univ, Affiliated Hosp 1, Guangzhou 510080, Peoples R China.
语种:
英文
关键词:
polyethylenimine;polyethylene glycol;isoporon diisocyanate;nonviral gene delivery;transfection
期刊:
Journal of Biomedical Materials Research - Part A
ISSN:
1549-3296
年:
2008
卷:
84
期:
3
页码:
795-804
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:材料科学;WOS学科类别:Engineering, Biomedical;Materials Science, Biomaterials
入藏号:
WOS:000252990200025;EI:20080811103249;PMID:17635020
机构署名:
本校为其他机构
院系归属:
生命科学与生物制药学院
摘要:
An ideal gene carrier is required both in safety and efficiency for transfection. Polyethylenimine (PEI), a well-studied cationic polymer, has been proved with high transfection efficiency, but is reported as toxicity in many cell lines. In this study, PEI was coupled with polyethylene glycol (PEG) to reduce its cytotoxicity. PEG–PEI copolymers were synthesized with isoporon diisocyanate (IPDI) in two steps. A set of PEG–PEI with different PEG molecular weights (MWs) and amounts of PEG were synthesized. The molecular structure of the resulting copolymers was evaluated by nuclear magnetic resonance spectroscopy (1H NMR), infrared spectroscopy (IR), and gel permeation chromatography (GPC), all of which had successfully verified formation of the copolymers. The particle size and zeta potential of polymer/DNA complexes were measured, and their cytotoxicity and transfection efficiency in Hela cells were evaluated. We found that the copolymer block structure significantly influenced not only the physicochemical properties of complexes, but also their cytotoxicity and transfection efficiency. PEG (5 kDa) significantly reduced the diameter of the spherical complexes. The zeta potential of complexes was reduced with increasing amount of PEG grafting. Cytotoxicity was dependent not on PEG MW but on the amount of PEG grafting. Copolymer PEG–PEI (2-25-1) with 1.89 PEG (2 kDa) was proved to be more efficient for in vitro gene transfer. In conclusion, PEG MW and the degree of PEGylation were found to significantly influence the biological activity of PEG–PEI/DNA complexes. These results provide new sights into the studies using block copolymer as gene delivery systems. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
参考文献:
Abdallah B, 1996, HUM GENE THER, V7, P1947, DOI 10.1089/hum.1996.7.16-1947
BOUSSIF O, 1995, P NATL ACAD SCI USA, V92, P7297, DOI 10.1073/pnas.92.16.7297
Collard WT, 2000, J PHARM SCI-US, V89, P499
Cook SE, 2005, J CONTROL RELEASE, V105, P151, DOI 10.1016/j.jconrel.2005.03.011
CRYSTAL RG, 1995, SCIENCE, V270, P404, DOI 10.1126/science.270.5235.404

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com