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Induction of Endoplasmic Reticulum Stress by Cadmium and Its Regulation on Nrf2 Signaling Pathway in Kidneys of Rats

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成果类型:
期刊论文
作者:
Chen Zhi Jian;Chen Jia Xing;Wu Li Kang*;Li Bi Yun;Tian Ya Feng;Xian Min;Huang Zi Pei;Yu Ri An*
通讯作者:
Yu Ri An;Wu Li Kang
作者机构:
[Chen Zhi Jian; Yu Ri An; Huang Zi Pei; Li Bi Yun; Chen Jia Xing] Guangdong Pharmaceut Univ, Sch Publ Hlth, Guangzhou 510310, Guangdong, Peoples R China.
[Xian Min; Wu Li Kang; Tian Ya Feng] Baoan Ctr Dis Control & Prevent Shenzhen, Shenzhen 518101, Guangdong, Peoples R China.
通讯机构:
[Yu Ri An] Guangdong Pharmaceut Univ, Sch Publ Hlth, Guangzhou 510310, Guangdong, Peoples R China.
[Wu Li Kang] Baoan Ctr Dis Control & Prevent Shenzhen, Shenzhen 518101, Guangdong, Peoples R China.
语种:
英文
关键词:
Cadmium;Endoplasmic reticulum stress;PERK;Nrf2;Kidney;Rat
期刊:
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
ISSN:
0895-3988
年:
2019
卷:
32
期:
1
页码:
1-10
文献类别:
WOS:Article
所属学科:
WOS学科类别:Environmental Sciences;Public, Environmental & Occupational Health
入藏号:
基金类别:
Science and Technology Plan Project of Shenzhen City in China [JCYJ20140414154847275]
机构署名:
本校为第一且通讯机构
院系归属:
公共卫生学院
摘要:
Objective This study was conducted to investigate the regulation of endoplasmic reticulum stress on Nrf2 signaling pathway in the kidneys of rats. Methods Rats were divided into twelve groups of six animals each. Some groups were pre-administered with bacitracin or tauroursodeoxycholic acid (TUDCA), and all of them were treated with 5-20 mu mol/kg cadmium (Cd) for 48 h. The oxidative stress levels were analyzed using kits. The mRNA and protein expression levels of endoplasmic reticulum stress-related factors and Nrf2 signaling pathway-related factors were determined using RT-PCR and western blot. Results Cd exposure resulted in oxidative stress in the kidneys of rats and upregulated the expression of endoplasmic reticulum stress (ERS)-related factors and Nrf2 signaling pathway-related factors, especially at doses of 10 and 20 mu mol/kg Cd, and the expression changes were particularly obvious. Moreover, after pretreatment with bacitracin, Cd upregulated the expression of ERS-related factors to a certain extent and, at higher doses, increased the mRNA expression of Nrf2. After pretreatment with TUDCA, Cd reduced the level of ERS to a certain extent; however, at these doses, there were no significant changes in the expression of Nrf2. Conclusion Cadmium can result in ERS and oxidative stress in the kidneys of rats, activate Nrf2, and upregulate the transcriptional expression of phase II detoxification enzymes under these experimental conditions. ERS has a positive regulation effect on Nrf2 signaling pathway but has little effect on the negative regulation of Nrf2 signaling pathway in cadmium toxicity.
参考文献:
Chen J, 2009, TOXICOL APPL PHARM, V241, P81, DOI 10.1016/j.taap.2009.07.038
Chen MH, 2017, ECOTOX ENVIRON SAFE, V145, P503, DOI 10.1016/j.ecoenv.2017.08.001
Chen S, 2014, NEUROPATH APPL NEURO, V40, P759, DOI 10.1111/nan.12103
[Hze Chen Zhijian], 2018, [N-р'ff�.
fH, Acta Medicinae Universitatis Scientiae et Technologiae Huazhong], V47, P59

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